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Neuron-Specific Enolase, Human, antibody

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Neuron-Specific Enolase, Human, antibody.  

Description: Neuron-Specific Enolase, Human, antibody.  

MoAb Isotype: IgG2a for MAbs 5E2, 1C1, 5A4, H11 and 2F1 IgG2b for MAb 5G10  

Clones Offered: 5G10, 5E2, 5A4, 1C1, H11, 2F1 Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with purified NSE from human brain.  

Specificity: Human NSE  

Applications: MAbs 5G10, 5A4 and 5E2 react with 45 kD band corresponding to NSE in immunoblotting of human brain extracts. MAbs may be used for immunohistochemistry on formalin-fixed and paraffin-embedded sections. Recommended dilution is 1:100 - 1:300. Optimal dilution must be determined by experimentation. All MAbs work in ELISA. Recommended pairs for sandwich immunoassay (coating - conjugate): 5G10 - 1C1 5E2 - 1C1 1C1 - 5E2 5A4 - 1C1 H11 - 2F1  

Purity: Chromatography on protein G Sepharose  

Presentation: PBS, pH 7.4, 0.1 % sodium azide (NaN3)  

Shipping Conditions: + 4 °C  

Comments: This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

Product Code: 2-NSE

Price: $312.00

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