Insulin, human, antibody

Product Information
Insulin, human, antibody.  

Description: Insulin, human, antibody.  

MoAb Isotype: IgG1, kappa for all MAbs  

Clones Offered: C7C9, D4B8, 7F8, 3A6, 8E2, 7F5 Hybridoma clones have been derived from hybridization of X63-Ag8-653 myeloma cells with spleen cells of Balb/c mice immunized with purified human insulin.  

Specificity: Insulin, human MAb C7C9 reacts with C-terminal pentapeptide of insulin beta-chain. MAbs 7F8, D4B8, 3A6 and 8E2 react with different antigenic determinants. Kass for MAb 7F8 is 7.5 x 109M-1 Kass for MAb D4B8 is 1.5 x 108M-1 Kass for MAb 3A6 is 8.0 x 108M-1 Kass for MAb 8E2 is 6.0 x 108M-1 Kass for MAb C7C9 is 1.0 x 109M-1 Kass for MAb 7F5 is 1.0 x 109M-1 These antibodies cross-react with human proinsulin, bovine insulin (30%) and porcine insulin. No cross-reaction with free C-peptide.  

Applications: Detection of insulin in two-site enzyme immunoassay. Following pairs are recommended (coating - conjugate): 7F8 - D4B8, 3A6 - 8E2. MAb D4B8 can be used in immunohistochemistry on frozen sections.  

Purity: Chromatography on protein A Sepharose  

Presentation: PBS, pH 7.4, 0.1 % sodium azide (NaN3)  

Shipping Conditions: + 4 °C  

Comments: This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

Price: $254.00

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