C-peptide, human, antibody

Product Information
C-peptide, human, antibody.  

Description: C-peptide, human, antibody.  

MoAb Isotype: IgG1, kappa for all MAbs  

Clones Offered: 1H8, 2B7, 4H8, 5B8, 7E10, 2A11 Hybridoma clones have been derived from hybridization of X63-Ag8-653 myeloma cells with spleen cells of Balb/c mice immunized with recombinant C-peptide construction.  

Specificity: No cross-reactivity with human, bovine, porcine and mouse/rat insulin. Affinity (Kd) > 1 x 108 M for all MAbs. All MAbs (except 2B7, 7E10 and 2A11) bind with equal efficiency to the human free C-peptide and C-peptide region in proinsulin molecules. Antigenic determinants identified by MAbs are in the three-dimensional structure generated by N- and C- terminal regions of C-peptide separated by beta-turn at position 47-50 of proinsulin.  

Applications: Competitive ELISA or RIA detection of C-peptide in human serum after immunospecific removal of proinsulin (with solid-phased 1D6 or 1D3 MAbs to human proinsulin). Construction of a sensitive two-site sandwich EIA/ RIA immunosorbent assay for the measurement of total human proinsulin (including proinsulin convertion intermediates) in serum. Specific immunosorbtional removal of total proinsulin and C-peptide molecules from serum samples (with solid-phase 1H8, 2B7 and 4H8) before the measurement of human insulin.  

Purity: Chromatography on protein A Sepharose  

Presentation: PBS, pH 7.4, 0.1 % sodium azide (NaN3)  

Shipping Conditions: + 4 °C  

Comments: This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

Price: $254.00

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